Electroporation system Gemini Twin Wave SC, Generator
Product information "Electroporation system Gemini Twin Wave SC, Generator"
The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.
Scope of supply: Generator Gemini SC
- Large, user-friendly touchscreen interface
- Universal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent forms
- Preset protocols include the most common eukaryotic and prokaryotic cell types
- User-defined protocols: protocols can be added and modified without limits
- Safety functions: Pre-pulse resistance check, arc protection, and over-current pulse abort
- Data protocols store parameters of all delivered pulses for quality control and troubleshooting purposes
- Storage of over 1000 protocols
| Exponential decay pulse | |
| Capacitance LV mode: | 25 ... 3275 µF |
| Capacitance HV mode: | 10, 25, 50 µF |
| Resistance: | 50 ... 1000 Ω |
| Voltage LV mode: | 10 ... 500 V |
| Voltage HV mode: | 510 ... 3000 V |
| Square wave pulse | |
| Capacitance LV mode: | 3775 µF |
| Capacitance HV mode: | 85 µF |
| Voltage LV mode: | 10 ... 500 V |
| Voltage HV mode: | 510 ... 3000 V |
| Multiple pulse LV mode: | 1 ... 10 pulses per sample |
| Multiple pulse HV mode: | 1 ... 2 pulses per sample |
| Pulse length LV mode: | 0.05 ... 100 ms |
| Pulse length HV mode: | 50 µs ... 5 ms |
| Pulse interval: | 0.1 ... 10 s |
| Charging time LV mode: | 7 s |
| Charging time HV mode: | 4 s |
| Depth: | 216 mm |
|---|---|
| Description: | Gemini SC Generator |
| Dimensions (WxDxH): | 286 x 216 x 324 mm |
| Height: | 324 mm |
| Manufacturer Article Number: | 452043 |
| Max. permitted ambient temperature: | 40 °C |
| Max. relative humidity rate: | 80 % |
| Min. permitted ambient temperature: | 4 °C |
| Permitted ambient conditions: | 4 ... 40 °C , 80 % RH |
| Power supply: | 100 ... 240 V, 50 ... 60 Hz |
| Type of auxiliary energy: | Mains connection |
| Weight: | 7.3 kg |
| Width: | 286 mm |
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Electroporation system Gemini Twin Wave SC, Complete system
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The Gemini Twin Wave devices are flexible systems that can generate both square wave and exponential decay waves. Ideally suited for all electroporation requirements. The waveform combination allows eukaryotic and prokaryotic cells to be electroporated easily and efficiently in cuvettes with a single device. Prokaryotic cells typically respond well to exponential decay wave pulses, while eukaryotic cells are most efficiently transfected with square wave pulses.Large, user-friendly touchscreen interfaceUniversal electroporation for transfection of cells in vitro, in vivo, in ovo, and adherent formsPreset protocols include the most common eukaryotic and prokaryotic cell typesUser-defined protocols: protocols can be added and modified without limitsSafety functions: Pre-pulse resistance check, arc protection, and over-current pulse abortData protocols store parameters of all delivered pulses for quality control and troubleshooting purposesStorage of over 1000 protocolsThe supplied safety dome connects to the generator to safely deliver high-voltage electrical pulses to cuvettes. Up to two cuvettes can be electroporated simultaneously.Scope of supply: Generator Gemini SC, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rackExponential decay pulseCapacitance LV mode:25 ... 3275 µFCapacitance HV mode:10, 25, 50 µFResistance:50 ... 1000 ΩVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VSquare wave pulseCapacitance LV mode:3775 µFCapacitance HV mode:85 µFVoltage LV mode:10 ... 500 VVoltage HV mode:510 ... 3000 VMultiple pulse LV mode:1 ... 100 pulses per sampleMultiple pulse HV mode:1 ... 2 pulses per samplePulse length LV mode:0.05 ... 100 msPulse length HV mode:50 µs ... 5 msPulse interval:0.1 ... 10 sCharging time LV mode:7 sCharging time HV mode:4 s
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Accessories
Electroporation cuvettes Plus
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BTX Cuvettes Plus are designed for use in electroporation and electrofusion of bacteria, yeast, insect, plant and mammalian cells. Each sterilized Cuvettes Plus package includes a disposable cuvette and a transfer pipette, which is used for removal of the sample after electroporation. The cuvettes are molded with embedded polished aluminum electrodes, and gamma irradiated for guaranteed sterility.Three electrode gap sizes are available:1mm for bacteria and yeast2mm for all cell types4mm for mammalian cellsBTX cuvette Plus can obtain high field strengths up to 25.0 kV/cm. Round caps are leak resistant and allow for quick and easy removal. For research use only. Can be used with electroporation systems from different manufacturers (not suitable for units from Eppendorf).