The Access RT-PCR System is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis Virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. The Access RT-PCR System includes an optimized single-buffer system that permits extremely sensitive detection of RNA transcripts without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples. In addition, the improved performance of AMV Reverse Transcriptase at elevated temperatures in the AMV/Tfl 5X Reaction Buffer minimizes problems encountered with secondary structures in RNA.

Column Wash Solution (CWA) is a component of the Wizard SV and Wizard SV 96 Genomic, Wizard Plus SV Minipreps and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems.

This simple, but elegant piece of equipment eliminates the need for centrifugation for elution of DNA when using the PureYield Midipreps or Maxiprep Plasmid DNA Purification Systems. The Eluator Vacuum Elution Device consists of two pieces, a blue base and a clear column assembly. The base interfaces with a Vacuum Manifold that contains Luer-Lok fittings, and holds a 1.5ml tube to capture eluted nucleic acids. The column assembly accepts PureYield Midiprep or Maxiprep columns. The Eluator Device not only saves time by eliminating the requirement for a centrifuge with a swinging bucket rotor, but also increases recovery of material compared to the centrifugation protocol.

The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15ug of plasmid DNA from 600ul to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TNT Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).